How to Establish MDD for Creams and Ophthalmic Solutions

[Naiffer_Host]

There is a previous discussion on analytical method for this NDSRI

Issue with Area Drop During N-Nitroso Dorzolamide Method Setup

I am currently setting up an analytical method for N-Nitroso Dorzolamide, but I am experiencing a significant drop in peak area values. Has anyone encountered this issue before? How did you resolve it? Please share your experience or suggestions.