Abstract
In this paper, the authors introduce a method that combines ion exchange chromatography with a easier-to-perform, simpler one-step post-column derivatization that is selective to nitrite and visible spectrophotometric detection to allow high sample loading volumes without affecting resolution. The method introduced here can be broadly applied to multiple polymeric excipient types. Ion-exchange chromatography post-column derivatization is used to determine ultra-low levels of nitrite in three excipients.
This method achieves nitrite measurement in excipients at the ppb level with IC and derivatization by Griess reagent.
