I’ve been given a method from a 3rd party to have a look at that is giving some unusual and variable results.
Nothing in it looks too unusual from other methods I have been looking at, except that any potential nitrosamine is washed from the SPE column utilising ammonia solution!! Now this by itself seems a little unusual, as other methods have involved aqueous formic acid, or formic acid in methanol.
I am wondering if there is the potential for the formation of nitrosamines during the preparation through the use of the ammonia and the presence of chloride ions, similar to below.
Does anyone have any thoughts, or seem anything similar?