@swaathi_1997 it seems you are working in positive mode. If it is, please switch to negative mode.
Negative mode which transition ?
@swaathi_1997 89 and 85
Even in negative mode same behaviour. area keeps changing.
which mobile phase have you used?
Any recommendation or contribution you can make to our discussion? That will be valuable for all of us.
Is the meglumine test also performed in the internal standard solution?