The question of peak tailing in the detection of the released N-NO of Nadroparinum calcicum by chemiluminescence

When we tested the N-NO group in nadroparin calcium with Ellutia CSS-TEA 810, we found that the peak of the sample tailed seriously, and it was not able to integrate normally, but the peak trailing of NDPA standard sample was not serious. According to EP, the limit of N-NO of Nadroparinum calcicumIs is NMT 0.25 PPM, the concentrations of NDPA standard samples were 0, 60, 120, 180ng/mL, the signal of stand solutions and samples was very low (all not more than 6 mv). Is it because of the low signal-noise ratio?

@AmandaGuiraldelli @giorgio